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Biotin Interference With Hormonal Assay
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I ran across something this morning that's important to know about. Biotin supplementation may falsely raise or lower many hormone assay results. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007242/ . The FDA has also issued several warnings. Tests can vary in their susceptibility to interference depending on manufacturer https://www.aacc.org/science-and-research/aacc-academy-guidance/biotin-interference-in-laboratory-tests . That article also describes the mechanisms of interference.
So far all information has pertained to human tests but most hormonal assays for horses are tests originally developed for humans then validated as working for horses. The susceptible methodologies described are used in many equine testing labs. In the absence of any equine specific information it would be wise to discontinue any supplements containing more than 2 mg of biotin for 6 days prior to hormone testing. -- Eleanor in PA www.drkellon.com
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HL's Mom
Dr.Kellon-
Thanks for the info. I don't give my horse anything with Biotin in it but I'll double check to see if any is in her feed. Diann Landau --- drkellon@... wrote: From: "Eleanor Kellon, VMD" <drkellon@...> To: main@ECIR.groups.io Subject: [Special] [ECIR] Biotin Interference With Hormonal Assay Date: Thu, 22 Oct 2020 07:36:10 -0700 I ran across something this morning that's important to knowabout. Biotin supplementation may falsely raise or lower many hormone assay results. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007242/ . The FDA has also issued several warnings. Tests can vary in their susceptibility to interference depending on manufacturer https://www.aacc.org/science-and-research/aacc-academy-guidance/ biotin-interference-in-laboratory-tests . That article also describes the mechanisms of interference. So far all information has pertained to human tests but most hormonal assays for horses are tests originally developed for humans then validated as working for horses. The susceptible methodologies described are used in many equine testing labs. In the absence of any equine specific information it would be wise to discontinue any supplements containing more than 2 mg of biotin for 6 days prior to hormone testing. -- Eleanor in PA www.drkellon.com EC Owner 2001 -- Diann in VA 2020
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Kate Schmidt-Hopper
Thanks for this info! Kate
On Thu, Oct 22, 2020 at 7:36 AM Eleanor Kellon, VMD <drkellon@...> wrote: I ran across something this morning that's important to know about. Biotin supplementation may falsely raise or lower many hormone assay results. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007242/ . The FDA has also issued several warnings. Tests can vary in their susceptibility to interference depending on manufacturer https://www.aacc.org/science-and-research/aacc-academy-guidance/biotin-interference-in-laboratory-tests . That article also describes the mechanisms of interference.
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Thank you Dr. Kellon
----- Original Message ----- From: Eleanor Kellon, VMD <drkellon@...> To: main@ECIR.groups.io Sent: Thu, 22 Oct 2020 10:36:10 -0400 (EDT) Subject: [Special] [ECIR] Biotin Interference With Hormonal Assay I ran across something this morning that's important to know about. Biotin supplementation may falsely raise or lower many hormone assay results. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007242/ . The FDA has also issued several warnings. Tests can vary in their susceptibility to interference depending on manufacturer https://www.aacc.org/science-and-research/aacc-academy-guidance/biotin-interference-in-laboratory-tests . That article also describes the mechanisms of interference. So far all information has pertained to human tests but most hormonal assays for horses are tests originally developed for humans then validated as working for horses. The susceptible methodologies described are used in many equine testing labs. In the absence of any equine specific information it would be wise to discontinue any supplements containing more than 2 mg of biotin for 6 days prior to hormone testing. -- Eleanor in PA www.drkellon.com -- - Frances C. December 2017, Washington & California Case history: https://ecir.groups.io/g/CaseHistory/files/Frances%20and%20Phoenix Phoenix's Photo Album: https://ecir.groups.io/g/CaseHistory/album?id=12382
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Valletta Lochridge
Thank you for this information, Dr Kellon. I want to be sure that I understand because I do add 20mg of Biotin to my mineral blend from Horse Tech. Currently, I don't have a horse with PPID but my 17 y/o percheron cross has compensated IR. As I understand the information in your post and in reading the articles, current thought is that Biotin supplementation isn't actually raising hormone levels in the body. If that's the case, the benefits are still worth adding it to the diet. Or, in your opinion, is there a question remaining as to the actual effect on hormone levels in the body? Do the benefits of Biotin outweigh the risk of information not yet known? Valletta in CA 2012
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Given that we don't know for sure yet if it's even a factor, and the alterations are not real - it's an artifact from the biotin, not a change in hormone levels - the benefits win out. I would say at this point it is mostly a consideration in scenarios where the results don't fit the clinical picture.
-- Eleanor in PA www.drkellon.com
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Valletta Lochridge
Wonderful - thank you, Dr Kellon. Valletta in CA 2012
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Thank you Dr. Kellon
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This might be a dumb question, but are we checking hormone levels when we test for PPID/IR? I never thought about it before I saw Dr Kellon's post.
-- Ronelle and Yoyo 2015 Bend, Or, US
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i Read the study on bio tin interference. I noticed the ladies on biotin,and we’re taking a very high doses. Is 20 mg for a horse a high enough dose to cause interference? thanks
-- LJ Friedman Nov 2014 Vista, Northern San Diego, CA Jesse and majestic ‘s Case History
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Interference has been reported with human doses as low as 5 mg, the equivalent of 20 mg for a horse.
-- Eleanor in PA www.drkellon.com
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Another hassle to get proper testing. Why don’t 20 of us do a test. Test acth and insulin with biotin and retest xxx number of days again, without biotin? II know my biotin is included in my custom supplement so that would be impossible to separate out.. but i could figure out something if others cant test. thoughts? thanks for posting about interference
-- LJ Friedman Nov 2014 Vista, Northern San Diego, CA Jesse and majestic ‘s Case History
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