Meal feeding before blood draw - clarification


Hilary McGregor
 

Perfect! Thanks so much for this detailed explanation and verification Kirsten! 

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Kirsten Rasmussen
 

Hi Hilary,

Yes, that's correct.

The recommendation to draw blood no less than 4 hours after breaking a fast (6+ hours without forage) is at least in part based on the results of this paper, which tracks postprandial (after an overnight fast) feeding changes in glucose and insulin.  You can see in Fig 3 that insulin normalizes between 180-240 minutes (3-4 hrs) after breakfast is fed depending on what was fed, regardless of how long it took to eat breakfast (less than 1 hr for hay breakfast: Fig 1), and it stays normalized/stable up to at least 360 minutes (6 hrs), which is as long as they measured.

I do not keep hay in front of my horse.  He finishes his breakfast in a slow feed net after about 3 hrs, and the vet usually draws blood between 4-5 hours after he had started his breakfast.  Our testing conditions are basically the same every time.

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Kirsten and Shaku (IR + PPID) - 2019
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Hilary McGregor
 

Correct, the hay takes until 8 am to finish, not the soy hulls which are gobbled in 1 minutes or so. 

So the guidance is: don't draw blood until 4 hours after breaking the fast/starting to eat?


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Hilary and Amika
Prince George, BC, Canada
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Sherry Morse
 

Hi Hilary,

Fasting bloodwork would be at least 6 hours without food in front of them.  If you feed concentrates and hay at the same time (7am) I would think the concentrates don't take an hour to eat and it's the hay you're referring to being finished at 8am.  Is that correct?  

Doing an 11- 11:30am blood draw, even if they finished everything by 8am - is fine as you're after the 4 hour mark from when the fast was broken.  

To be 100% sure things were being done as correctly as possible I'd try to schedule afternoon blood draws later but again, 4 hours is not considered fasting for a horse which has quite a long digestive tract.

Hope that helps.




Hilary McGregor
 

Good morning. I've read and re-read the directions for non-fasted insulin/glucose testing. However, I'm wondering if I may have misinterpreted the directions.

I feed at 7:00 am hay and minerals in small soy hull mash. They finish eating around 8:00am. They are fed hay again at noon, 4 hours after finishing breakfast. I cannot keep hay in front of them, they will eat their day's ration in 4 hours.

I've understood that I could draw blood after 11:00 am and avoid the insulin spike, however now I'm wondering if it's 4 hours after they finish eating?? Somehow that direction doesn't make sense if a horse is a slow eater, or if you are following the direction to keep hay in front of them until blood draw. Seems like the clear direction would be a certain number of hours after breaking the overnight fast i.e. x hours from when the first meal is started.

We just drew blood at noon before feeding lunch. In the past we've done it at 1:30pm just after lunch. I believe we've also done it at 11:30am before. Are these all likely to provide comparable results, or no?

Please help me understand the nuances here! 
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Hilary and Amika
Prince George, BC, Canada
Joined July 2019
NRC+ proficient grad January 2022

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